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Temperature dependence of ssrA-tag mediated protein degradation

Building synthetic gene networks with highly transient dynamics requires rapid protein degradation. We show that the degradation conferred by two commonly used ssrA tags is highly temperature dependent. Synthetic gene networks are being used increasingly in real-world applications where they may be...

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Detalles Bibliográficos
Autores principales: Purcell, Oliver, Grierson, Claire S, Bernardo, Mario di, Savery, Nigel J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3511798/
https://www.ncbi.nlm.nih.gov/pubmed/22824000
http://dx.doi.org/10.1186/1754-1611-6-10