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Temperature dependence of ssrA-tag mediated protein degradation
Building synthetic gene networks with highly transient dynamics requires rapid protein degradation. We show that the degradation conferred by two commonly used ssrA tags is highly temperature dependent. Synthetic gene networks are being used increasingly in real-world applications where they may be...
Autores principales: | Purcell, Oliver, Grierson, Claire S, Bernardo, Mario di, Savery, Nigel J |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3511798/ https://www.ncbi.nlm.nih.gov/pubmed/22824000 http://dx.doi.org/10.1186/1754-1611-6-10 |
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