A novel system for stable, high-level expression from the T7 promoter

BACKGROUND: The most widespread, efficient prokaryotic protein-producing system is one where the T7 phage polymerase recognizes the T7 phage promoter (T7 p/p system). Unfortunately, in this system, target protein expression gradually declines and is often undetectable following 3 to 5 subcultures. A...

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Detalles Bibliográficos
Autores principales: Kesik-Brodacka, Malgorzata, Romanik, Agnieszka, Mikiewicz-Sygula, Diana, Plucienniczak, Grazyna, Plucienniczak, Andrzej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3512507/
https://www.ncbi.nlm.nih.gov/pubmed/22897977
http://dx.doi.org/10.1186/1475-2859-11-109

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3512507/
https://www.ncbi.nlm.nih.gov/pubmed/22897977
http://dx.doi.org/10.1186/1475-2859-11-109

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