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Does limited virucidal activity of biocides include duck hepatitis B virucidal action?

BACKGROUND: There is agreement that the infectivity assay with the duck hepatitis B virus (DHBV) is a suitable surrogate test to validate disinfectants for hepatitis B virucidal activity. However, since this test is not widely used, information is necessary whether disinfectants with limited virucid...

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Autores principales: Sauerbrei, Andreas, Schacke, Michael, Glück, Brigitte, Bust, Uwe, Rabenau, Holger F, Wutzler, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514261/
https://www.ncbi.nlm.nih.gov/pubmed/23110658
http://dx.doi.org/10.1186/1471-2334-12-276
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author Sauerbrei, Andreas
Schacke, Michael
Glück, Brigitte
Bust, Uwe
Rabenau, Holger F
Wutzler, Peter
author_facet Sauerbrei, Andreas
Schacke, Michael
Glück, Brigitte
Bust, Uwe
Rabenau, Holger F
Wutzler, Peter
author_sort Sauerbrei, Andreas
collection PubMed
description BACKGROUND: There is agreement that the infectivity assay with the duck hepatitis B virus (DHBV) is a suitable surrogate test to validate disinfectants for hepatitis B virucidal activity. However, since this test is not widely used, information is necessary whether disinfectants with limited virucidal activity also inactivate DHBV. In general, disinfectants with limited virucidal activity are used for skin and sensitive surfaces while agents with full activity are more aggressive. The present study compares the activity of five different biocides against DHBV and the classical test virus for limited virucidal activity, the vaccinia virus strain Lister Elstree (VACV) or the modified vaccinia Ankara strain (MVA). METHODS: Virucidal assay was performed as suspension test according to the German DVV/RKI guideline. Duck hepatitis B virus obtained from congenitally infected Peking ducks was propagated in primary duck embryonic hepatocytes and was detected by indirect immunofluorescent antigen staining. RESULTS: The DHBV was inactivated by the use of 40% ethanol within 1-min and 30% isopropanol within 2-min exposure. In comparison, 40% ethanol within 2-min and 40% isopropanol within 1-min exposure were effective against VACV/MVA. These alcohols only have limited virucidal activity, while the following agents have full activity. 0.01% peracetic acid inactivated DHBV within 2 min and a concentration of 0.005% had virucidal efficacy against VACV/MVA within 1 min. After 2-min exposure, 0.05% glutardialdehyde showed a comparable activity against DHBV and VACV/MVA. This is also the case for 0.7% formaldehyde after a contact time of 30 min. CONCLUSIONS: Duck hepatitis B virus is at least as sensitive to limited virucidal activity as VACV/MVA. Peracetic acid is less effective against DHBV, while the alcohols are less effective against VACV/MVA. It can be expected that in absence of more direct tests the results may be extrapolated to HBV.
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spelling pubmed-35142612012-12-05 Does limited virucidal activity of biocides include duck hepatitis B virucidal action? Sauerbrei, Andreas Schacke, Michael Glück, Brigitte Bust, Uwe Rabenau, Holger F Wutzler, Peter BMC Infect Dis Research Article BACKGROUND: There is agreement that the infectivity assay with the duck hepatitis B virus (DHBV) is a suitable surrogate test to validate disinfectants for hepatitis B virucidal activity. However, since this test is not widely used, information is necessary whether disinfectants with limited virucidal activity also inactivate DHBV. In general, disinfectants with limited virucidal activity are used for skin and sensitive surfaces while agents with full activity are more aggressive. The present study compares the activity of five different biocides against DHBV and the classical test virus for limited virucidal activity, the vaccinia virus strain Lister Elstree (VACV) or the modified vaccinia Ankara strain (MVA). METHODS: Virucidal assay was performed as suspension test according to the German DVV/RKI guideline. Duck hepatitis B virus obtained from congenitally infected Peking ducks was propagated in primary duck embryonic hepatocytes and was detected by indirect immunofluorescent antigen staining. RESULTS: The DHBV was inactivated by the use of 40% ethanol within 1-min and 30% isopropanol within 2-min exposure. In comparison, 40% ethanol within 2-min and 40% isopropanol within 1-min exposure were effective against VACV/MVA. These alcohols only have limited virucidal activity, while the following agents have full activity. 0.01% peracetic acid inactivated DHBV within 2 min and a concentration of 0.005% had virucidal efficacy against VACV/MVA within 1 min. After 2-min exposure, 0.05% glutardialdehyde showed a comparable activity against DHBV and VACV/MVA. This is also the case for 0.7% formaldehyde after a contact time of 30 min. CONCLUSIONS: Duck hepatitis B virus is at least as sensitive to limited virucidal activity as VACV/MVA. Peracetic acid is less effective against DHBV, while the alcohols are less effective against VACV/MVA. It can be expected that in absence of more direct tests the results may be extrapolated to HBV. BioMed Central 2012-10-30 /pmc/articles/PMC3514261/ /pubmed/23110658 http://dx.doi.org/10.1186/1471-2334-12-276 Text en Copyright ©2012 Sauerbrei et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sauerbrei, Andreas
Schacke, Michael
Glück, Brigitte
Bust, Uwe
Rabenau, Holger F
Wutzler, Peter
Does limited virucidal activity of biocides include duck hepatitis B virucidal action?
title Does limited virucidal activity of biocides include duck hepatitis B virucidal action?
title_full Does limited virucidal activity of biocides include duck hepatitis B virucidal action?
title_fullStr Does limited virucidal activity of biocides include duck hepatitis B virucidal action?
title_full_unstemmed Does limited virucidal activity of biocides include duck hepatitis B virucidal action?
title_short Does limited virucidal activity of biocides include duck hepatitis B virucidal action?
title_sort does limited virucidal activity of biocides include duck hepatitis b virucidal action?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514261/
https://www.ncbi.nlm.nih.gov/pubmed/23110658
http://dx.doi.org/10.1186/1471-2334-12-276
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