Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase – engineering a thermostable ATP independent enzyme

BACKGROUND: RNA ligases are essential reagents for many methods in molecular biology including NextGen RNA sequencing. To prevent ligation of RNA to itself, ATP independent mutant ligases, defective in self-adenylation, are often used in combination with activated pre-adenylated linkers. It is impor...

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Detalles Bibliográficos
Autores principales: Zhelkovsky, Alexander M, McReynolds, Larry A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514331/
https://www.ncbi.nlm.nih.gov/pubmed/22809063
http://dx.doi.org/10.1186/1471-2199-13-24

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514331/
https://www.ncbi.nlm.nih.gov/pubmed/22809063
http://dx.doi.org/10.1186/1471-2199-13-24

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