Quantification of Reverse Transcriptase Activity by Real-Time PCR as a Fast and Accurate Method for Titration of HIV, Lenti- and Retroviral Vectors

Quantification of retroviruses in cell culture supernatants and other biological preparations is required in a diverse spectrum of laboratories and applications. Methods based on antigen detection, such as p24 for HIV, or on genome detection are virus specific and sometimes suffer from a limited dyn...

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Detalles Bibliográficos
Autores principales: Vermeire, Jolien, Naessens, Evelien, Vanderstraeten, Hanne, Landi, Alessia, Iannucci, Veronica, Van Nuffel, Anouk, Taghon, Tom, Pizzato, Massimo, Verhasselt, Bruno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3515444/
https://www.ncbi.nlm.nih.gov/pubmed/23227216
http://dx.doi.org/10.1371/journal.pone.0050859

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3515444/
https://www.ncbi.nlm.nih.gov/pubmed/23227216
http://dx.doi.org/10.1371/journal.pone.0050859

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