A Recombinant Protein Based on Trypanosoma cruzi P21 Enhances Phagocytosis

BACKGROUND: P21 is a secreted protein expressed in all developmental stages of Trypanosoma cruzi. The aim of this study was to determine the effect of the recombinant protein based on P21 (P21-His(6)) on inflammatory macrophages during phagocytosis. FINDINGS: Our results showed that P21-His(6) acts...

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Autores principales: Rodrigues, Adele A., Clemente, Tatiana M., dos Santos, Marlus A., Machado, Fabrício C., Gomes, Rafael G. B., Moreira, Heline Hellen T., Cruz, Mário C., Brígido, Paula C., dos Santos, Paulo C. F., Martins, Flávia A., Bahia, Diana, Maricato, Juliana T., Janini, Luiz M. R., Reboredo, Eduardo H., Mortara, Renato A., da Silva, Claudio V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3519637/
https://www.ncbi.nlm.nih.gov/pubmed/23251513
http://dx.doi.org/10.1371/journal.pone.0051384
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author Rodrigues, Adele A.
Clemente, Tatiana M.
dos Santos, Marlus A.
Machado, Fabrício C.
Gomes, Rafael G. B.
Moreira, Heline Hellen T.
Cruz, Mário C.
Brígido, Paula C.
dos Santos, Paulo C. F.
Martins, Flávia A.
Bahia, Diana
Maricato, Juliana T.
Janini, Luiz M. R.
Reboredo, Eduardo H.
Mortara, Renato A.
da Silva, Claudio V.
author_facet Rodrigues, Adele A.
Clemente, Tatiana M.
dos Santos, Marlus A.
Machado, Fabrício C.
Gomes, Rafael G. B.
Moreira, Heline Hellen T.
Cruz, Mário C.
Brígido, Paula C.
dos Santos, Paulo C. F.
Martins, Flávia A.
Bahia, Diana
Maricato, Juliana T.
Janini, Luiz M. R.
Reboredo, Eduardo H.
Mortara, Renato A.
da Silva, Claudio V.
author_sort Rodrigues, Adele A.
collection PubMed
description BACKGROUND: P21 is a secreted protein expressed in all developmental stages of Trypanosoma cruzi. The aim of this study was to determine the effect of the recombinant protein based on P21 (P21-His(6)) on inflammatory macrophages during phagocytosis. FINDINGS: Our results showed that P21-His(6) acts as a phagocytosis inducer by binding to CXCR4 chemokine receptor and activating actin polymerization in a way dependent onthe PI3-kinase signaling pathway. CONCLUSIONS: Thus, our results shed light on the notion that native P21 is a component related to T. cruzi evasion from the immune response and that CXCR4 may be involved in phagocytosis. P21-His(6) represents an important experimental control tool to study phagocytosis signaling pathways of different intracellular parasites and particles.
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spelling pubmed-35196372012-12-18 A Recombinant Protein Based on Trypanosoma cruzi P21 Enhances Phagocytosis Rodrigues, Adele A. Clemente, Tatiana M. dos Santos, Marlus A. Machado, Fabrício C. Gomes, Rafael G. B. Moreira, Heline Hellen T. Cruz, Mário C. Brígido, Paula C. dos Santos, Paulo C. F. Martins, Flávia A. Bahia, Diana Maricato, Juliana T. Janini, Luiz M. R. Reboredo, Eduardo H. Mortara, Renato A. da Silva, Claudio V. PLoS One Research Article BACKGROUND: P21 is a secreted protein expressed in all developmental stages of Trypanosoma cruzi. The aim of this study was to determine the effect of the recombinant protein based on P21 (P21-His(6)) on inflammatory macrophages during phagocytosis. FINDINGS: Our results showed that P21-His(6) acts as a phagocytosis inducer by binding to CXCR4 chemokine receptor and activating actin polymerization in a way dependent onthe PI3-kinase signaling pathway. CONCLUSIONS: Thus, our results shed light on the notion that native P21 is a component related to T. cruzi evasion from the immune response and that CXCR4 may be involved in phagocytosis. P21-His(6) represents an important experimental control tool to study phagocytosis signaling pathways of different intracellular parasites and particles. Public Library of Science 2012-12-10 /pmc/articles/PMC3519637/ /pubmed/23251513 http://dx.doi.org/10.1371/journal.pone.0051384 Text en © 2012 Rodrigues et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Rodrigues, Adele A.
Clemente, Tatiana M.
dos Santos, Marlus A.
Machado, Fabrício C.
Gomes, Rafael G. B.
Moreira, Heline Hellen T.
Cruz, Mário C.
Brígido, Paula C.
dos Santos, Paulo C. F.
Martins, Flávia A.
Bahia, Diana
Maricato, Juliana T.
Janini, Luiz M. R.
Reboredo, Eduardo H.
Mortara, Renato A.
da Silva, Claudio V.
A Recombinant Protein Based on Trypanosoma cruzi P21 Enhances Phagocytosis
title A Recombinant Protein Based on Trypanosoma cruzi P21 Enhances Phagocytosis
title_full A Recombinant Protein Based on Trypanosoma cruzi P21 Enhances Phagocytosis
title_fullStr A Recombinant Protein Based on Trypanosoma cruzi P21 Enhances Phagocytosis
title_full_unstemmed A Recombinant Protein Based on Trypanosoma cruzi P21 Enhances Phagocytosis
title_short A Recombinant Protein Based on Trypanosoma cruzi P21 Enhances Phagocytosis
title_sort recombinant protein based on trypanosoma cruzi p21 enhances phagocytosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3519637/
https://www.ncbi.nlm.nih.gov/pubmed/23251513
http://dx.doi.org/10.1371/journal.pone.0051384
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