IL-27 increases BST-2 expression in human monocytes and T cells independently of type I IFN
IL-27 modulates inflammatory responses by influencing cytokine secretion and CD4 T cell differentiation. Recently, IL-27 was demonstrated to inhibit HIV replication by inducing type I interferon (IFN) expression and subsequent IFN-dependent expression of apolipoprotein B mRNA-editing enzyme, catalyt...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521153/ https://www.ncbi.nlm.nih.gov/pubmed/23240078 http://dx.doi.org/10.1038/srep00974 |
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author | Guzzo, Christina Jung, Masany Graveline, Ashley Banfield, Bruce W. Gee, Katrina |
author_facet | Guzzo, Christina Jung, Masany Graveline, Ashley Banfield, Bruce W. Gee, Katrina |
author_sort | Guzzo, Christina |
collection | PubMed |
description | IL-27 modulates inflammatory responses by influencing cytokine secretion and CD4 T cell differentiation. Recently, IL-27 was demonstrated to inhibit HIV replication by inducing type I interferon (IFN) expression and subsequent IFN-dependent expression of apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like (APOBEC)-3 family members, a group of antiviral cytidine deaminases. To characterize other anti-viral genes modulated by IL-27, we examined another IFN-responsive gene: tetherin/bone marrow stromal cell antigen 2 (BST-2). Our study shows that IL-27 can directly induce BST-2 expression, independently of an intermediary type I IFN response. Quantitative RT-PCR analysis demonstrated IL-27-induced BST-2 mRNA expression as early as 2h after exposure of cells to IL-27. In the presence of the type I IFN-neutralizing protein, B18R, IL-27-induced BST-2 expression was maintained, demonstrating that IFN is not an intermediary in IL-27-induced BST-2. Taken together, our findings identify a novel function of IL-27 as a direct stimulator of BST-2 expression. |
format | Online Article Text |
id | pubmed-3521153 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-35211532012-12-13 IL-27 increases BST-2 expression in human monocytes and T cells independently of type I IFN Guzzo, Christina Jung, Masany Graveline, Ashley Banfield, Bruce W. Gee, Katrina Sci Rep Article IL-27 modulates inflammatory responses by influencing cytokine secretion and CD4 T cell differentiation. Recently, IL-27 was demonstrated to inhibit HIV replication by inducing type I interferon (IFN) expression and subsequent IFN-dependent expression of apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like (APOBEC)-3 family members, a group of antiviral cytidine deaminases. To characterize other anti-viral genes modulated by IL-27, we examined another IFN-responsive gene: tetherin/bone marrow stromal cell antigen 2 (BST-2). Our study shows that IL-27 can directly induce BST-2 expression, independently of an intermediary type I IFN response. Quantitative RT-PCR analysis demonstrated IL-27-induced BST-2 mRNA expression as early as 2h after exposure of cells to IL-27. In the presence of the type I IFN-neutralizing protein, B18R, IL-27-induced BST-2 expression was maintained, demonstrating that IFN is not an intermediary in IL-27-induced BST-2. Taken together, our findings identify a novel function of IL-27 as a direct stimulator of BST-2 expression. Nature Publishing Group 2012-12-13 /pmc/articles/PMC3521153/ /pubmed/23240078 http://dx.doi.org/10.1038/srep00974 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Guzzo, Christina Jung, Masany Graveline, Ashley Banfield, Bruce W. Gee, Katrina IL-27 increases BST-2 expression in human monocytes and T cells independently of type I IFN |
title | IL-27 increases BST-2 expression in human monocytes and T cells independently of type I IFN |
title_full | IL-27 increases BST-2 expression in human monocytes and T cells independently of type I IFN |
title_fullStr | IL-27 increases BST-2 expression in human monocytes and T cells independently of type I IFN |
title_full_unstemmed | IL-27 increases BST-2 expression in human monocytes and T cells independently of type I IFN |
title_short | IL-27 increases BST-2 expression in human monocytes and T cells independently of type I IFN |
title_sort | il-27 increases bst-2 expression in human monocytes and t cells independently of type i ifn |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521153/ https://www.ncbi.nlm.nih.gov/pubmed/23240078 http://dx.doi.org/10.1038/srep00974 |
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