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Methanol immersion reduces spherical aberration of water dipping lenses at long wavelengths used in multi-photon laser scanning microscopy

Dipping objectives were tested for multi-photon laser scanning microscopy, since their large working distances are advantageous for thick specimens and the absence of a coverslip facilitates examination of living material. Images of fluorescent bead specimens, particularly at wavelengths greater tha...

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Detalles Bibliográficos
Autores principales: Norris, Greg, Gebril, Ayman, Ferro, Valerie A., McConnell, Gail
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521300/
https://www.ncbi.nlm.nih.gov/pubmed/23243580
http://dx.doi.org/10.1364/BOE.3.003314