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Lentiviral transduction of rat Sertoli cells as a means to modify gene expression
Primary cell culture is an established and widely used technique to study Sertoli cell function in vitro. However, the relative difficulty of stably overexpressing or knocking down genes in Sertoli cell culture has limited progress in the field. In this technical report, we present a method to trans...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Landes Bioscience
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521750/ https://www.ncbi.nlm.nih.gov/pubmed/23248769 http://dx.doi.org/10.4161/spmg.22516 |