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Lentiviral transduction of rat Sertoli cells as a means to modify gene expression

Primary cell culture is an established and widely used technique to study Sertoli cell function in vitro. However, the relative difficulty of stably overexpressing or knocking down genes in Sertoli cell culture has limited progress in the field. In this technical report, we present a method to trans...

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Detalles Bibliográficos
Autores principales: Nicholls, Peter K., Stanton, Peter G., Rainczuk, Katarzyna E., Qian, Hongwei, Gregorevic, Paul, Harrison, Craig A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521750/
https://www.ncbi.nlm.nih.gov/pubmed/23248769
http://dx.doi.org/10.4161/spmg.22516