Anti-PML-RARα shRNA sensitises promyelocytic leukaemia cells to all-trans retinoic acid

The PML-RARα fusion gene disrupts the retinoic acid differentiation signal in a range of leukaemia types, promoting proliferation. We designed a shRNA to target the fusion mRNA, and the shRNA expression cassette was delivered via lentiviral transduction. Delivery of this shRNA significantly down reg...

Descripción completa

Detalles Bibliográficos
Autores principales: Casey, Nicholas P, Woods, Gregory M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of RNAi and Gene Silencing 2012
Materias:
Research Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3522483/
https://www.ncbi.nlm.nih.gov/pubmed/23300507
Descripción
Sumario:The PML-RARα fusion gene disrupts the retinoic acid differentiation signal in a range of leukaemia types, promoting proliferation. We designed a shRNA to target the fusion mRNA, and the shRNA expression cassette was delivered via lentiviral transduction. Delivery of this shRNA significantly down regulated the target mRNA, with effects also evident at the protein level. When combined with ATRA administration, this down regulation of the fusion gene strongly inhibited proliferation in the NB4 PML cell line.

Ejemplares similares