Anti-PML-RARα shRNA sensitises promyelocytic leukaemia cells to all-trans retinoic acid

The PML-RARα fusion gene disrupts the retinoic acid differentiation signal in a range of leukaemia types, promoting proliferation. We designed a shRNA to target the fusion mRNA, and the shRNA expression cassette was delivered via lentiviral transduction. Delivery of this shRNA significantly down reg...

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Detalles Bibliográficos
Autores principales: Casey, Nicholas P, Woods, Gregory M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of RNAi and Gene Silencing 2012
Materias:
Research Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3522483/
https://www.ncbi.nlm.nih.gov/pubmed/23300507
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author Casey, Nicholas P
Woods, Gregory M
author_facet Casey, Nicholas P
Woods, Gregory M
author_sort Casey, Nicholas P
collection PubMed
description The PML-RARα fusion gene disrupts the retinoic acid differentiation signal in a range of leukaemia types, promoting proliferation. We designed a shRNA to target the fusion mRNA, and the shRNA expression cassette was delivered via lentiviral transduction. Delivery of this shRNA significantly down regulated the target mRNA, with effects also evident at the protein level. When combined with ATRA administration, this down regulation of the fusion gene strongly inhibited proliferation in the NB4 PML cell line.
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spelling pubmed-35224832013-01-08 Anti-PML-RARα shRNA sensitises promyelocytic leukaemia cells to all-trans retinoic acid Casey, Nicholas P Woods, Gregory M J RNAi Gene Silencing Research Report The PML-RARα fusion gene disrupts the retinoic acid differentiation signal in a range of leukaemia types, promoting proliferation. We designed a shRNA to target the fusion mRNA, and the shRNA expression cassette was delivered via lentiviral transduction. Delivery of this shRNA significantly down regulated the target mRNA, with effects also evident at the protein level. When combined with ATRA administration, this down regulation of the fusion gene strongly inhibited proliferation in the NB4 PML cell line. Journal of RNAi and Gene Silencing 2012-07-13 /pmc/articles/PMC3522483/ /pubmed/23300507 Text en © Copyright The Author(s) http://creativecommons.org/licenses/by-nc/2.5 Published by Library Publishing Media. This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5). This license permits non-commercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details.
spellingShingle Research Report
Casey, Nicholas P
Woods, Gregory M
Anti-PML-RARα shRNA sensitises promyelocytic leukaemia cells to all-trans retinoic acid
title Anti-PML-RARα shRNA sensitises promyelocytic leukaemia cells to all-trans retinoic acid
title_full Anti-PML-RARα shRNA sensitises promyelocytic leukaemia cells to all-trans retinoic acid
title_fullStr Anti-PML-RARα shRNA sensitises promyelocytic leukaemia cells to all-trans retinoic acid
title_full_unstemmed Anti-PML-RARα shRNA sensitises promyelocytic leukaemia cells to all-trans retinoic acid
title_short Anti-PML-RARα shRNA sensitises promyelocytic leukaemia cells to all-trans retinoic acid
title_sort anti-pml-rarα shrna sensitises promyelocytic leukaemia cells to all-trans retinoic acid
topic Research Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3522483/
https://www.ncbi.nlm.nih.gov/pubmed/23300507
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AT woodsgregorym antipmlrarashrnasensitisespromyelocyticleukaemiacellstoalltransretinoicacid

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