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Monitoring Intracellular Calcium Ion Dynamics in Hair Cell Populations with Fluo-4 AM

We optimized Fluo-4 AM loading of chicken cochlea to report hair-bundle Ca(2+) signals in populations of hair cells. The bundle Ca(2+) signal reported the physiological state of the bundle and cell; extruding cells had very high bundle Fluo-4 fluorescence, cells with intact bundles and tip links had...

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Detalles Bibliográficos
Autores principales: Spinelli, Kateri J., Gillespie, Peter G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524081/
https://www.ncbi.nlm.nih.gov/pubmed/23284798
http://dx.doi.org/10.1371/journal.pone.0051874