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Monitoring Intracellular Calcium Ion Dynamics in Hair Cell Populations with Fluo-4 AM
We optimized Fluo-4 AM loading of chicken cochlea to report hair-bundle Ca(2+) signals in populations of hair cells. The bundle Ca(2+) signal reported the physiological state of the bundle and cell; extruding cells had very high bundle Fluo-4 fluorescence, cells with intact bundles and tip links had...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524081/ https://www.ncbi.nlm.nih.gov/pubmed/23284798 http://dx.doi.org/10.1371/journal.pone.0051874 |