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PFunkel: Efficient, Expansive, User-Defined Mutagenesis
We introduce PFunkel, a versatile method for extensive, researcher-defined DNA mutagenesis using a ssDNA or dsDNA template. Once the template DNA is prepared, the method can be completed in a single day in a single tube, and requires no intermediate DNA purification or sub-cloning. PFunkel can be us...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2012
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524131/ https://www.ncbi.nlm.nih.gov/pubmed/23284860 http://dx.doi.org/10.1371/journal.pone.0052031 |