Relation between expression pattern of p53 and survivin in cutaneous basal cell carcinomas

BACKGROUND: The tumor suppressor gene p53 is a key regulator of cell division and/or apoptosis. Survivin is a multifunctional member of the inhibitor of apoptosis family. Survivin and p53 represent diametrically opposed signals that influence the apoptotic pathway. MATERIAL/METHODS: To determine the...

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Autores principales: Adamkov, Marian, Halasova, Erika, Rajcani, Julius, Bencat, Marian, Vybohova, Desanka, Rybarova, Silvia, Galbavy, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524735/
https://www.ncbi.nlm.nih.gov/pubmed/21358596
http://dx.doi.org/10.12659/MSM.881442
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author Adamkov, Marian
Halasova, Erika
Rajcani, Julius
Bencat, Marian
Vybohova, Desanka
Rybarova, Silvia
Galbavy, Stefan
author_facet Adamkov, Marian
Halasova, Erika
Rajcani, Julius
Bencat, Marian
Vybohova, Desanka
Rybarova, Silvia
Galbavy, Stefan
author_sort Adamkov, Marian
collection PubMed
description BACKGROUND: The tumor suppressor gene p53 is a key regulator of cell division and/or apoptosis. Survivin is a multifunctional member of the inhibitor of apoptosis family. Survivin and p53 represent diametrically opposed signals that influence the apoptotic pathway. MATERIAL/METHODS: To determine the role of p53 and survivin in basal cell carcinoma (BCC), we evaluated the expression pattern of both proteins with regard to the percentage of positively immunostained tumor cells, the intensity of staining, and subcellular localization among 31 subjects with BCC. RESULTS: Overexpression of p53 protein was found in 28 of 31 cases (90.3%), whereas survivin accumulation was seen in 27 (87.1%). For p53, moderate and/or strong immunoreactivity was seen in 20 of 28 cases (71.4%), and 26 of 28 cases (92.9%) showed more than 25% reactive tumor cells. Nuclear p53 staining was detected in 23 of 28 cases (82.1%), whereas combined nuclear and cytoplasmic localization was found in only 5 of 28 cases (17.9%). Survivin revealed mild intensity of immuno-reaction in 22 of 27 cases (71%), and 25 of 27 cases (92.6%) showed less than 25% labeled tumor cells. Combined nuclear and cytoplasmic survivin localization was present in 26 of 27 cases (96.3%). Statistically significant differences were detected in the assessed expression parameters between those proteins. CONCLUSIONS: Our results suggest that overexpression of wild type p53 protein may suppress the expression of survivin and its antiapoptotic activity in BCC cells.
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spelling pubmed-35247352013-04-24 Relation between expression pattern of p53 and survivin in cutaneous basal cell carcinomas Adamkov, Marian Halasova, Erika Rajcani, Julius Bencat, Marian Vybohova, Desanka Rybarova, Silvia Galbavy, Stefan Med Sci Monit Basic Research BACKGROUND: The tumor suppressor gene p53 is a key regulator of cell division and/or apoptosis. Survivin is a multifunctional member of the inhibitor of apoptosis family. Survivin and p53 represent diametrically opposed signals that influence the apoptotic pathway. MATERIAL/METHODS: To determine the role of p53 and survivin in basal cell carcinoma (BCC), we evaluated the expression pattern of both proteins with regard to the percentage of positively immunostained tumor cells, the intensity of staining, and subcellular localization among 31 subjects with BCC. RESULTS: Overexpression of p53 protein was found in 28 of 31 cases (90.3%), whereas survivin accumulation was seen in 27 (87.1%). For p53, moderate and/or strong immunoreactivity was seen in 20 of 28 cases (71.4%), and 26 of 28 cases (92.9%) showed more than 25% reactive tumor cells. Nuclear p53 staining was detected in 23 of 28 cases (82.1%), whereas combined nuclear and cytoplasmic localization was found in only 5 of 28 cases (17.9%). Survivin revealed mild intensity of immuno-reaction in 22 of 27 cases (71%), and 25 of 27 cases (92.6%) showed less than 25% labeled tumor cells. Combined nuclear and cytoplasmic survivin localization was present in 26 of 27 cases (96.3%). Statistically significant differences were detected in the assessed expression parameters between those proteins. CONCLUSIONS: Our results suggest that overexpression of wild type p53 protein may suppress the expression of survivin and its antiapoptotic activity in BCC cells. International Scientific Literature, Inc. 2011-03-01 /pmc/articles/PMC3524735/ /pubmed/21358596 http://dx.doi.org/10.12659/MSM.881442 Text en © Med Sci Monit, 2011 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
spellingShingle Basic Research
Adamkov, Marian
Halasova, Erika
Rajcani, Julius
Bencat, Marian
Vybohova, Desanka
Rybarova, Silvia
Galbavy, Stefan
Relation between expression pattern of p53 and survivin in cutaneous basal cell carcinomas
title Relation between expression pattern of p53 and survivin in cutaneous basal cell carcinomas
title_full Relation between expression pattern of p53 and survivin in cutaneous basal cell carcinomas
title_fullStr Relation between expression pattern of p53 and survivin in cutaneous basal cell carcinomas
title_full_unstemmed Relation between expression pattern of p53 and survivin in cutaneous basal cell carcinomas
title_short Relation between expression pattern of p53 and survivin in cutaneous basal cell carcinomas
title_sort relation between expression pattern of p53 and survivin in cutaneous basal cell carcinomas
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524735/
https://www.ncbi.nlm.nih.gov/pubmed/21358596
http://dx.doi.org/10.12659/MSM.881442
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