Single nucleotide polymorphism genotyping by two colour melting curve analysis using the MGB Eclipse™ Probe System in challenging sequence environment

Probe and primer design for single nucleotide polymorphism (SNP) detection can be very challenging for A-T DNA-rich targets, requiring long sequences with lower specificity and stability, while G-C-rich DNA targets present limited design options to lower GC-content sequences only. We have developed...

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Detalles Bibliográficos
Autores principales: Belousov, Yevgeniy S, Welch, Robert A, Sanders, Silvia, Mills, Alan, Kulchenko, Alena, Dempcy, Robert, Afonina, Irina A, Walburger, David K, Glaser, Cynthia L, Yadavalli, Sunita, Vermeulen, Nicolaas MJ, Mahoney, Walt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Primary Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3525082/
https://www.ncbi.nlm.nih.gov/pubmed/15588480
http://dx.doi.org/10.1186/1479-7364-1-3-209

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3525082/
https://www.ncbi.nlm.nih.gov/pubmed/15588480
http://dx.doi.org/10.1186/1479-7364-1-3-209

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