Cargando…

Most Anti-BrdU Antibodies React with 2′-Deoxy-5-Ethynyluridine — The Method for the Effective Suppression of This Cross-Reactivity

5-Bromo-2′-deoxyuridine (BrdU) and 2′-deoxy-5-ethynyluridine (EdU) are widely used as markers of replicated DNA. While BrdU is detected using antibodies, the click reaction typically with fluorescent azido-dyes is used for EdU localisation. We have performed an analysis of ten samples of antibodies...

Descripción completa

Detalles Bibliográficos
Autores principales: Liboska, Radek, Ligasová, Anna, Strunin, Dmytro, Rosenberg, Ivan, Koberna, Karel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3525573/
https://www.ncbi.nlm.nih.gov/pubmed/23272138
http://dx.doi.org/10.1371/journal.pone.0051679