Cargando…
Most Anti-BrdU Antibodies React with 2′-Deoxy-5-Ethynyluridine — The Method for the Effective Suppression of This Cross-Reactivity
5-Bromo-2′-deoxyuridine (BrdU) and 2′-deoxy-5-ethynyluridine (EdU) are widely used as markers of replicated DNA. While BrdU is detected using antibodies, the click reaction typically with fluorescent azido-dyes is used for EdU localisation. We have performed an analysis of ten samples of antibodies...
Autores principales: | Liboska, Radek, Ligasová, Anna, Strunin, Dmytro, Rosenberg, Ivan, Koberna, Karel |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3525573/ https://www.ncbi.nlm.nih.gov/pubmed/23272138 http://dx.doi.org/10.1371/journal.pone.0051679 |
Ejemplares similares
-
Atomic Scissors: A New Method of Tracking the 5-Bromo-2′-Deoxyuridine-Labeled DNA In Situ
por: Ligasová, Anna, et al.
Publicado: (2012) -
Looking for ugly ducklings: The role of the stability of BrdU-antibody complex and the improved method of the detection of DNA replication
por: Ligasová, Anna, et al.
Publicado: (2017) -
The Fingerprint of Anti-Bromodeoxyuridine Antibodies and Its Use for the Assessment of Their Affinity to 5-Bromo-2'-Deoxyuridine in Cellular DNA under Various Conditions
por: Ligasová, Anna, et al.
Publicado: (2015) -
A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA
por: Ligasová, Anna, et al.
Publicado: (2013) -
Unexpected BrdU inhibition on astrocyte-to-neuron conversion
por: Wang, Tao, et al.
Publicado: (2021)