Manipulating replisome dynamics to enhance lambda Red-mediated multiplex genome engineering

Disrupting the interaction between primase and helicase in Escherichia coli increases Okazaki fragment (OF) length due to less frequent primer synthesis. We exploited this feature to increase the amount of ssDNA at the lagging strand of the replication fork that is available for λ Red-mediated Multi...

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Detalles Bibliográficos
Autores principales: Lajoie, M. J., Gregg, C. J., Mosberg, J. A., Washington, G. C., Church, G. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3526312/
https://www.ncbi.nlm.nih.gov/pubmed/22904085
http://dx.doi.org/10.1093/nar/gks751

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3526312/
https://www.ncbi.nlm.nih.gov/pubmed/22904085
http://dx.doi.org/10.1093/nar/gks751

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