Similar Gene Estimates from Circular and Linear Standards in Quantitative PCR Analyses Using the Prokaryotic 16S rRNA Gene as a Model

Quantitative PCR (qPCR) is one of the most widely used tools for quantifying absolute numbers of microbial gene copies in test samples. A recent publication showed that circular plasmid DNA standards grossly overestimated numbers of a target gene by as much as 8-fold in a eukaryotic system using qua...

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Detalles Bibliográficos
Autores principales: Oldham, Athenia L., Duncan, Kathleen E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3526484/
https://www.ncbi.nlm.nih.gov/pubmed/23284822
http://dx.doi.org/10.1371/journal.pone.0051931

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3526484/
https://www.ncbi.nlm.nih.gov/pubmed/23284822
http://dx.doi.org/10.1371/journal.pone.0051931

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