“Direct cloning in Lactobacillus plantarum: Electroporation with non-methylated plasmid DNA enhances transformation efficiency and makes shuttle vectors obsolete”

BACKGROUND: Lactic acid bacteria (LAB) play an important role in agricultural as well as industrial biotechnology. Development of improved LAB strains using e.g. library approaches is often limited by low transformation efficiencies wherefore one reason could be differences in the DNA methylation pa...

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Detalles Bibliográficos
Autores principales: Spath, Katharina, Heinl, Stefan, Grabherr, Reingard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3526553/
https://www.ncbi.nlm.nih.gov/pubmed/23098256
http://dx.doi.org/10.1186/1475-2859-11-141

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3526553/
https://www.ncbi.nlm.nih.gov/pubmed/23098256
http://dx.doi.org/10.1186/1475-2859-11-141

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