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Non-rigid image registration to reduce beam-induced blurring of cryo-electron microscopy images

The typical dose used to record cryo-electron microscopy images from vitrified biological specimens is so high that radiation-induced structural alterations are bound to occur during data acquisition. Integration of all scattered electrons into one image can lead to significant blurring, particularl...

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Autores principales: Karimi Nejadasl, Fatemeh, Karuppasamy, Manikandan, Newman, Emily R., McGeehan, John E., Ravelli, Raimond B. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3526921/
https://www.ncbi.nlm.nih.gov/pubmed/23254656
http://dx.doi.org/10.1107/S0909049512044408
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author Karimi Nejadasl, Fatemeh
Karuppasamy, Manikandan
Newman, Emily R.
McGeehan, John E.
Ravelli, Raimond B. G.
author_facet Karimi Nejadasl, Fatemeh
Karuppasamy, Manikandan
Newman, Emily R.
McGeehan, John E.
Ravelli, Raimond B. G.
author_sort Karimi Nejadasl, Fatemeh
collection PubMed
description The typical dose used to record cryo-electron microscopy images from vitrified biological specimens is so high that radiation-induced structural alterations are bound to occur during data acquisition. Integration of all scattered electrons into one image can lead to significant blurring, particularly if the data are collected from an unsupported thin layer of ice suspended over the holes of a support film. Here, the dose has been fractioned and exposure series have been acquired in order to study beam-induced specimen movements under low dose conditions, prior to bubbling. Gold particles were added to the protein sample as fiducial markers. These were automatically localized and tracked throughout the exposure series and showed correlated motions within small patches, with larger amplitudes of motion vectors at the start of a series compared with the end of each series. A non-rigid scheme was used to register all images within each exposure series, using natural neighbor interpolation with the gold particles as anchor points. The procedure increases the contrast and resolution of the examined macromolecules.
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spelling pubmed-35269212013-01-09 Non-rigid image registration to reduce beam-induced blurring of cryo-electron microscopy images Karimi Nejadasl, Fatemeh Karuppasamy, Manikandan Newman, Emily R. McGeehan, John E. Ravelli, Raimond B. G. J Synchrotron Radiat Radiation Damage The typical dose used to record cryo-electron microscopy images from vitrified biological specimens is so high that radiation-induced structural alterations are bound to occur during data acquisition. Integration of all scattered electrons into one image can lead to significant blurring, particularly if the data are collected from an unsupported thin layer of ice suspended over the holes of a support film. Here, the dose has been fractioned and exposure series have been acquired in order to study beam-induced specimen movements under low dose conditions, prior to bubbling. Gold particles were added to the protein sample as fiducial markers. These were automatically localized and tracked throughout the exposure series and showed correlated motions within small patches, with larger amplitudes of motion vectors at the start of a series compared with the end of each series. A non-rigid scheme was used to register all images within each exposure series, using natural neighbor interpolation with the gold particles as anchor points. The procedure increases the contrast and resolution of the examined macromolecules. International Union of Crystallography 2013-01-01 2012-11-29 /pmc/articles/PMC3526921/ /pubmed/23254656 http://dx.doi.org/10.1107/S0909049512044408 Text en © Fatemeh Karimi Nejadasl et al. 2013 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Radiation Damage
Karimi Nejadasl, Fatemeh
Karuppasamy, Manikandan
Newman, Emily R.
McGeehan, John E.
Ravelli, Raimond B. G.
Non-rigid image registration to reduce beam-induced blurring of cryo-electron microscopy images
title Non-rigid image registration to reduce beam-induced blurring of cryo-electron microscopy images
title_full Non-rigid image registration to reduce beam-induced blurring of cryo-electron microscopy images
title_fullStr Non-rigid image registration to reduce beam-induced blurring of cryo-electron microscopy images
title_full_unstemmed Non-rigid image registration to reduce beam-induced blurring of cryo-electron microscopy images
title_short Non-rigid image registration to reduce beam-induced blurring of cryo-electron microscopy images
title_sort non-rigid image registration to reduce beam-induced blurring of cryo-electron microscopy images
topic Radiation Damage
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3526921/
https://www.ncbi.nlm.nih.gov/pubmed/23254656
http://dx.doi.org/10.1107/S0909049512044408
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