A set of dual promoter vectors for high throughput cloning, screening, and protein expression in eukaryotic and prokaryotic systems from a single plasmid

BACKGROUND: The ability to produce the same recombinant protein in both prokaryotic and eukaryotic cells offers many experimental opportunities. However, the cloning of the same gene into multiple plasmids is required, which is time consuming, laborious and still may not produce soluble, stable prot...

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Detalles Bibliográficos
Autores principales: Sinah, Namita, Williams, Charlotte A, Piper, Robert C, Shields, S Brookhart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527174/
https://www.ncbi.nlm.nih.gov/pubmed/22916790
http://dx.doi.org/10.1186/1472-6750-12-54

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527174/
https://www.ncbi.nlm.nih.gov/pubmed/22916790
http://dx.doi.org/10.1186/1472-6750-12-54

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