Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins

Insoluble recombinant proteins are a major issue for both structural genomics and enzymology research. Greater than 30% of recombinant proteins expressed in Escherichia coli (E. coli) appear to be insoluble. The prevailing view is that insolubly expressed proteins cannot be easily solubilized, and a...

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Detalles Bibliográficos
Autores principales: Leibly, David J., Nguyen, Trang Nhu, Kao, Louis T., Hewitt, Stephen N., Barrett, Lynn K., Van Voorhis, Wesley C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527557/
https://www.ncbi.nlm.nih.gov/pubmed/23285060
http://dx.doi.org/10.1371/journal.pone.0052482

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527557/
https://www.ncbi.nlm.nih.gov/pubmed/23285060
http://dx.doi.org/10.1371/journal.pone.0052482

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