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Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins
Insoluble recombinant proteins are a major issue for both structural genomics and enzymology research. Greater than 30% of recombinant proteins expressed in Escherichia coli (E. coli) appear to be insoluble. The prevailing view is that insolubly expressed proteins cannot be easily solubilized, and a...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527557/ https://www.ncbi.nlm.nih.gov/pubmed/23285060 http://dx.doi.org/10.1371/journal.pone.0052482 |