An Intramolecular G-Quadruplex Structure Is Required for Binding of Telomeric Repeat-Containing RNA to the Telomeric Protein TRF2

[Image: see text] Telomeric repeat-containing RNA (TERRA) is important for telomere regulation, but the structural basis for how TERRA localizes to chromosome ends is unknown. Here we report on studies exploring whether the TERRA G-quadruplex structure is critical for binding to telomeres. We demons...

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Autores principales: Biffi, Giulia, Tannahill, David, Balasubramanian, Shankar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2012
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528108/
https://www.ncbi.nlm.nih.gov/pubmed/22780456
http://dx.doi.org/10.1021/ja305734x
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author Biffi, Giulia
Tannahill, David
Balasubramanian, Shankar
author_facet Biffi, Giulia
Tannahill, David
Balasubramanian, Shankar
author_sort Biffi, Giulia
collection PubMed
description [Image: see text] Telomeric repeat-containing RNA (TERRA) is important for telomere regulation, but the structural basis for how TERRA localizes to chromosome ends is unknown. Here we report on studies exploring whether the TERRA G-quadruplex structure is critical for binding to telomeres. We demonstrate that the telomeric protein TRF2 binds TERRA via interactions that necessitate the formation of a G-quadruplex structure rather than the TERRA sequence per se. We also show that TRF2 simultaneously binds TERRA and telomeric duplex or G-quadruplex DNA. These observations suggest that the TERRA G-quadruplex is a key feature of telomere organization.
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spelling pubmed-35281082012-12-21 An Intramolecular G-Quadruplex Structure Is Required for Binding of Telomeric Repeat-Containing RNA to the Telomeric Protein TRF2 Biffi, Giulia Tannahill, David Balasubramanian, Shankar J Am Chem Soc [Image: see text] Telomeric repeat-containing RNA (TERRA) is important for telomere regulation, but the structural basis for how TERRA localizes to chromosome ends is unknown. Here we report on studies exploring whether the TERRA G-quadruplex structure is critical for binding to telomeres. We demonstrate that the telomeric protein TRF2 binds TERRA via interactions that necessitate the formation of a G-quadruplex structure rather than the TERRA sequence per se. We also show that TRF2 simultaneously binds TERRA and telomeric duplex or G-quadruplex DNA. These observations suggest that the TERRA G-quadruplex is a key feature of telomere organization. American Chemical Society 2012-07-10 2012-07-25 /pmc/articles/PMC3528108/ /pubmed/22780456 http://dx.doi.org/10.1021/ja305734x Text en Copyright © 2012 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.
spellingShingle Biffi, Giulia
Tannahill, David
Balasubramanian, Shankar
An Intramolecular G-Quadruplex Structure Is Required for Binding of Telomeric Repeat-Containing RNA to the Telomeric Protein TRF2
title An Intramolecular G-Quadruplex Structure Is Required for Binding of Telomeric Repeat-Containing RNA to the Telomeric Protein TRF2
title_full An Intramolecular G-Quadruplex Structure Is Required for Binding of Telomeric Repeat-Containing RNA to the Telomeric Protein TRF2
title_fullStr An Intramolecular G-Quadruplex Structure Is Required for Binding of Telomeric Repeat-Containing RNA to the Telomeric Protein TRF2
title_full_unstemmed An Intramolecular G-Quadruplex Structure Is Required for Binding of Telomeric Repeat-Containing RNA to the Telomeric Protein TRF2
title_short An Intramolecular G-Quadruplex Structure Is Required for Binding of Telomeric Repeat-Containing RNA to the Telomeric Protein TRF2
title_sort intramolecular g-quadruplex structure is required for binding of telomeric repeat-containing rna to the telomeric protein trf2
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528108/
https://www.ncbi.nlm.nih.gov/pubmed/22780456
http://dx.doi.org/10.1021/ja305734x
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