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Construction of pET-32 α (+) Vector for Protein Expression and Purification

The construction of expression vector is a basic tool for biotechnology and production of desired proteins, this article summarized the construction of pET-32 α (+) vector techniques which are generally used in research laboratories. The procedures include that acquisition of the exogenous DNA fragm...

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Detalles Bibliográficos
Autores principales: Liu, Zhi-Qiang, Yang, Ping-Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3530323/
https://www.ncbi.nlm.nih.gov/pubmed/23272309
http://dx.doi.org/10.4103/1947-2714.104318