Naringenin Decreases Invasiveness and Metastasis by Inhibiting TGF-β-Induced Epithelial to Mesenchymal Transition in Pancreatic Cancer Cells

Epithelial to mesenchymal transition (EMT) promotes cellular motility, invasiveness and metastasis during embryonic development and tumorigenesis. Transforming growth factor-β (TGF-β) signaling pathway is a key regulator of EMT. A lot of evidences suggest that this process is Smad3-dependent. Herein...

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Autores principales: Lou, Changjie, Zhang, Fayun, Yang, Ming, Zhao, Juan, Zeng, Wenfeng, Fang, Xiaocui, Zhang, Yanqiao, Zhang, Chunling, Liang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3530567/
https://www.ncbi.nlm.nih.gov/pubmed/23300530
http://dx.doi.org/10.1371/journal.pone.0050956
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author Lou, Changjie
Zhang, Fayun
Yang, Ming
Zhao, Juan
Zeng, Wenfeng
Fang, Xiaocui
Zhang, Yanqiao
Zhang, Chunling
Liang, Wei
author_facet Lou, Changjie
Zhang, Fayun
Yang, Ming
Zhao, Juan
Zeng, Wenfeng
Fang, Xiaocui
Zhang, Yanqiao
Zhang, Chunling
Liang, Wei
author_sort Lou, Changjie
collection PubMed
description Epithelial to mesenchymal transition (EMT) promotes cellular motility, invasiveness and metastasis during embryonic development and tumorigenesis. Transforming growth factor-β (TGF-β) signaling pathway is a key regulator of EMT. A lot of evidences suggest that this process is Smad3-dependent. Herein we showed that exposure of aspc-1 and panc-1 pancreatic cancer cells to TGF-β1 resulted in characteristic morphological alterations of EMT, and enhancement of cell motility and gemcitabine (Gem) resistance along with an up-regulation of EMT markers genes such as vimentin, N-cadherin, MMP2 and MMP9. Naringenin (Nar) down-regulated EMT markers expression in both mRNA and protein levels by inhibiting TGF-β1/Smad3 signal pathway in the pancreatic cancer cells. Consequently, Nar suppressed the cells migration and invasion and reversed their resistance to Gem.
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spelling pubmed-35305672013-01-08 Naringenin Decreases Invasiveness and Metastasis by Inhibiting TGF-β-Induced Epithelial to Mesenchymal Transition in Pancreatic Cancer Cells Lou, Changjie Zhang, Fayun Yang, Ming Zhao, Juan Zeng, Wenfeng Fang, Xiaocui Zhang, Yanqiao Zhang, Chunling Liang, Wei PLoS One Research Article Epithelial to mesenchymal transition (EMT) promotes cellular motility, invasiveness and metastasis during embryonic development and tumorigenesis. Transforming growth factor-β (TGF-β) signaling pathway is a key regulator of EMT. A lot of evidences suggest that this process is Smad3-dependent. Herein we showed that exposure of aspc-1 and panc-1 pancreatic cancer cells to TGF-β1 resulted in characteristic morphological alterations of EMT, and enhancement of cell motility and gemcitabine (Gem) resistance along with an up-regulation of EMT markers genes such as vimentin, N-cadherin, MMP2 and MMP9. Naringenin (Nar) down-regulated EMT markers expression in both mRNA and protein levels by inhibiting TGF-β1/Smad3 signal pathway in the pancreatic cancer cells. Consequently, Nar suppressed the cells migration and invasion and reversed their resistance to Gem. Public Library of Science 2012-12-26 /pmc/articles/PMC3530567/ /pubmed/23300530 http://dx.doi.org/10.1371/journal.pone.0050956 Text en © 2012 Lou et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lou, Changjie
Zhang, Fayun
Yang, Ming
Zhao, Juan
Zeng, Wenfeng
Fang, Xiaocui
Zhang, Yanqiao
Zhang, Chunling
Liang, Wei
Naringenin Decreases Invasiveness and Metastasis by Inhibiting TGF-β-Induced Epithelial to Mesenchymal Transition in Pancreatic Cancer Cells
title Naringenin Decreases Invasiveness and Metastasis by Inhibiting TGF-β-Induced Epithelial to Mesenchymal Transition in Pancreatic Cancer Cells
title_full Naringenin Decreases Invasiveness and Metastasis by Inhibiting TGF-β-Induced Epithelial to Mesenchymal Transition in Pancreatic Cancer Cells
title_fullStr Naringenin Decreases Invasiveness and Metastasis by Inhibiting TGF-β-Induced Epithelial to Mesenchymal Transition in Pancreatic Cancer Cells
title_full_unstemmed Naringenin Decreases Invasiveness and Metastasis by Inhibiting TGF-β-Induced Epithelial to Mesenchymal Transition in Pancreatic Cancer Cells
title_short Naringenin Decreases Invasiveness and Metastasis by Inhibiting TGF-β-Induced Epithelial to Mesenchymal Transition in Pancreatic Cancer Cells
title_sort naringenin decreases invasiveness and metastasis by inhibiting tgf-β-induced epithelial to mesenchymal transition in pancreatic cancer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3530567/
https://www.ncbi.nlm.nih.gov/pubmed/23300530
http://dx.doi.org/10.1371/journal.pone.0050956
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