Express Your LOV: An Engineered Flavoprotein as a Reporter for Protein Expression and Purification

In this work, we describe the utility of Light, Oxygen, or Voltage-sensing (LOV) flavoprotein domains from plant phototropins as a reporter for protein expression and function. Specifically, we used iLOV, an enhanced and more photostable variant of LOV. A pET-based plasmid for protein expression was...

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Detalles Bibliográficos
Autores principales: Gawthorne, Jayde A., Reddick, L. Evan, Akpunarlieva, Snezhana N., Beckham, Katherine S. H., Christie, John M., Alto, Neal M., Gabrielsen, Mads, Roe, Andrew J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531456/
https://www.ncbi.nlm.nih.gov/pubmed/23300834
http://dx.doi.org/10.1371/journal.pone.0052962

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531456/
https://www.ncbi.nlm.nih.gov/pubmed/23300834
http://dx.doi.org/10.1371/journal.pone.0052962

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