Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing

High-throughput sequencing has revolutionized microbial ecology, but read quality remains a significant barrier to accurate taxonomy assignment and alpha diversity assessment for microbial communities. We demonstrate that high-quality read length and abundance are the primary factors differentiating...

Descripción completa

Detalles Bibliográficos
Autores principales: Bokulich, Nicholas A., Subramanian, Sathish, Faith, Jeremiah J., Gevers, Dirk, Gordon, Jeffrey I., Knight, Rob, Mills, David A., Caporaso, J. Gregory
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531572/
https://www.ncbi.nlm.nih.gov/pubmed/23202435
http://dx.doi.org/10.1038/nmeth.2276

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531572/
https://www.ncbi.nlm.nih.gov/pubmed/23202435
http://dx.doi.org/10.1038/nmeth.2276

Ejemplares similares