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Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing
High-throughput sequencing has revolutionized microbial ecology, but read quality remains a significant barrier to accurate taxonomy assignment and alpha diversity assessment for microbial communities. We demonstrate that high-quality read length and abundance are the primary factors differentiating...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531572/ https://www.ncbi.nlm.nih.gov/pubmed/23202435 http://dx.doi.org/10.1038/nmeth.2276 |