Digestion and depletion of abundant proteins improves proteomic coverage

Two major challenges in proteomics are the large number of proteins and their broad dynamic range within the cell. We exploited the abundance-dependent Michaelis-Menten kinetics of trypsin digestion to selectively digest and deplete abundant proteins with a method we call DigDeAPr. We validated the...

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Detalles Bibliográficos
Autores principales: Fonslow, Bryan R., Stein, Benjamin D., Webb, Kristofor J., Xu, Tao, Choi, Jeong, Park, Sung Kyu, Yates, John R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531578/
https://www.ncbi.nlm.nih.gov/pubmed/23160281
http://dx.doi.org/10.1038/nmeth.2250

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531578/
https://www.ncbi.nlm.nih.gov/pubmed/23160281
http://dx.doi.org/10.1038/nmeth.2250

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