Exponential Megapriming PCR (EMP) Cloning—Seamless DNA Insertion into Any Target Plasmid without Sequence Constraints

We present a fast, reliable and inexpensive restriction-free cloning method for seamless DNA insertion into any plasmid without sequence limitation. Exponential megapriming PCR (EMP) cloning requires two consecutive PCR steps and can be carried out in one day. We show that EMP cloning has a higher e...

Descripción completa

Detalles Bibliográficos
Autores principales: Ulrich, Alexander, Andersen, Kasper R., Schwartz, Thomas U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534072/
https://www.ncbi.nlm.nih.gov/pubmed/23300917
http://dx.doi.org/10.1371/journal.pone.0053360

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534072/
https://www.ncbi.nlm.nih.gov/pubmed/23300917
http://dx.doi.org/10.1371/journal.pone.0053360

Ejemplares similares