Fusion of the Dhfr/Mtx and IR/MAR Gene Amplification Methods Produces a Rapid and Efficient Method for Stable Recombinant Protein Production

Amplification of the dihydrofolate reductase gene (Dhfr) by methotrexate (Mtx) exposure is commonly used for recombinant protein expression in Chinese hamster ovary (CHO) cells. However, this method is both time- and labor-intensive, and the high-producing cells that are generated are frequently uns...

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Detalles Bibliográficos
Autores principales: Noguchi, Chiemi, Araki, Yoshio, Miki, Daisuke, Shimizu, Noriaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534112/
https://www.ncbi.nlm.nih.gov/pubmed/23300841
http://dx.doi.org/10.1371/journal.pone.0052990

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534112/
https://www.ncbi.nlm.nih.gov/pubmed/23300841
http://dx.doi.org/10.1371/journal.pone.0052990

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