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Improved base-calling and quality scores for 454 sequencing based on a Hurdle Poisson model

BACKGROUND: 454 pyrosequencing is a commonly used massively parallel DNA sequencing technology with a wide variety of application fields such as epigenetics, metagenomics and transcriptomics. A well-known problem of this platform is its sensitivity to base-calling insertion and deletion errors, part...

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Detalles Bibliográficos
Autores principales: Beuf, Kristof De, Schrijver, Joachim De, Thas, Olivier, Criekinge, Wim Van, Irizarry, Rafael A, Clement, Lieven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534400/
https://www.ncbi.nlm.nih.gov/pubmed/23151247
http://dx.doi.org/10.1186/1471-2105-13-303