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LNA probes substantially improve the detection of bacterial endosymbionts in whole mount of insects by fluorescent in-situ hybridization

BACKGROUND: Detection of unculturable bacteria and their localization in the host, by fluorescent in-situ hybridization (FISH), is a powerful technique in the study of host-bacteria interaction. FISH probes are designed to target the 16 s rRNA region of the bacteria to be detected. LNA probes have r...

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Detalles Bibliográficos
Autores principales: Priya, Natarajan Gayatri, Pandey, Neeti, Rajagopal, Raman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3536699/
https://www.ncbi.nlm.nih.gov/pubmed/22624773
http://dx.doi.org/10.1186/1471-2180-12-81