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In vitro substrate phosphorylation by Ca(2+)/calmodulin-dependent protein kinase kinase using guanosine-5(′)-triphosphate as a phosphate donor
BACKGROUND: Ca(2+)/calmodulin-dependent protein kinase kinase (CaMKK) phosphorylates and activates particular downstream protein kinases — including CaMKI, CaMKIV, and AMPK— to stimulate multiple Ca(2+)-signal transduction pathways. To identify previously unidentified CaMKK substrates, we used vario...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3537517/ https://www.ncbi.nlm.nih.gov/pubmed/23216827 http://dx.doi.org/10.1186/1471-2091-13-27 |