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Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green

cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H(2)O(2) over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H(2)O(2) improved the coupled reac...

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Detalles Bibliográficos
Autores principales: Son, Yu-Lim, Kim, Hyoun-Young, Thiyagarajan, Saravanakumar, Xu, Jing Jing, Park, Seung-Moon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3538974/
https://www.ncbi.nlm.nih.gov/pubmed/23323052
http://dx.doi.org/10.5941/MYCO.2012.40.4.258