Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green

cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H(2)O(2) over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H(2)O(2) improved the coupled reac...

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Autores principales: Son, Yu-Lim, Kim, Hyoun-Young, Thiyagarajan, Saravanakumar, Xu, Jing Jing, Park, Seung-Moon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2012
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3538974/
https://www.ncbi.nlm.nih.gov/pubmed/23323052
http://dx.doi.org/10.5941/MYCO.2012.40.4.258
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author Son, Yu-Lim
Kim, Hyoun-Young
Thiyagarajan, Saravanakumar
Xu, Jing Jing
Park, Seung-Moon
author_facet Son, Yu-Lim
Kim, Hyoun-Young
Thiyagarajan, Saravanakumar
Xu, Jing Jing
Park, Seung-Moon
author_sort Son, Yu-Lim
collection PubMed
description cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H(2)O(2) over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H(2)O(2) improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150 µM within 90 min.
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spelling pubmed-35389742013-01-15 Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green Son, Yu-Lim Kim, Hyoun-Young Thiyagarajan, Saravanakumar Xu, Jing Jing Park, Seung-Moon Mycobiology Research Note cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H(2)O(2) over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H(2)O(2) improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150 µM within 90 min. The Korean Society of Mycology 2012-12 2012-12-26 /pmc/articles/PMC3538974/ /pubmed/23323052 http://dx.doi.org/10.5941/MYCO.2012.40.4.258 Text en © The Korean Society of Mycology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Note
Son, Yu-Lim
Kim, Hyoun-Young
Thiyagarajan, Saravanakumar
Xu, Jing Jing
Park, Seung-Moon
Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green
title Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green
title_full Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green
title_fullStr Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green
title_full_unstemmed Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green
title_short Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green
title_sort heterologous expression of phanerochaete chrysoporium glyoxal oxidase and its application for the coupled reaction with manganese peroxidase to decolorize malachite green
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3538974/
https://www.ncbi.nlm.nih.gov/pubmed/23323052
http://dx.doi.org/10.5941/MYCO.2012.40.4.258
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