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Activation of the Renal PI3K/Akt/mTOR Signaling Pathway in a DOCA-Salt Model of Hypertension

The present study investigated the changes that occurred in the mammalian target of rapamycin (mTOR) signaling pathway in the kidney as a result of deoxycorticosterone acetate (DOCA)-salt hypertension. Rats were implanted with DOCA strips (200 mg/kg) 1 week after unilateral nephrectomy and were then...

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Autores principales: Ma, Seong Kwon, Choi, Joon Seok, Joo, Soo Yeon, Kim, Ha Yeon, Kim, Chang Seong, Bae, Eun Hui, Lee, Jong Un, Kim, Soo Wan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chonnam National University Medical School 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539094/
https://www.ncbi.nlm.nih.gov/pubmed/23323219
http://dx.doi.org/10.4068/cmj.2012.48.3.150
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author Ma, Seong Kwon
Choi, Joon Seok
Joo, Soo Yeon
Kim, Ha Yeon
Kim, Chang Seong
Bae, Eun Hui
Lee, Jong Un
Kim, Soo Wan
author_facet Ma, Seong Kwon
Choi, Joon Seok
Joo, Soo Yeon
Kim, Ha Yeon
Kim, Chang Seong
Bae, Eun Hui
Lee, Jong Un
Kim, Soo Wan
author_sort Ma, Seong Kwon
collection PubMed
description The present study investigated the changes that occurred in the mammalian target of rapamycin (mTOR) signaling pathway in the kidney as a result of deoxycorticosterone acetate (DOCA)-salt hypertension. Rats were implanted with DOCA strips (200 mg/kg) 1 week after unilateral nephrectomy and were then supplied with 0.9% saline to drink. Four weeks after DOCA implantation, systolic blood pressure (SBP) was measured by use of the tail-cuff method. The expression levels of phosphorylated phosphatidylinositol-3-kinase (PI3K), Akt, and mTOR, as well as the protein expression levels of ED-1 and cyclooxygenase-2 (COX-2), transforming growth factor-β1 (TGF-β1), α-smooth muscle actin (SMA), caspase-3, Bax, and Bcl-2, were then examined in the kidney by semiquantitative immunoblotting. DOCA-salt hypertensive rats were found to have significantly increased SBP as well as an increased kidney weight-to-body weight ratio. Moreover, the phosphorylation of PI3K, Akt, and mTOR was increased in the kidney of DOCA-salt hypertensive rats compared with the control, as was the protein expression of ED-1, COX-2, TGF-β1, and α-SMA. The expression levels of caspase-3 and Bax were increased significantly, whereas Bcl-2 expression was decreased. In conclusion, the phosphorylation of PI3K/Akt/mTOR was increased in the kidney of DOCA-salt hypertensive rats.
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spelling pubmed-35390942013-01-15 Activation of the Renal PI3K/Akt/mTOR Signaling Pathway in a DOCA-Salt Model of Hypertension Ma, Seong Kwon Choi, Joon Seok Joo, Soo Yeon Kim, Ha Yeon Kim, Chang Seong Bae, Eun Hui Lee, Jong Un Kim, Soo Wan Chonnam Med J Original Article The present study investigated the changes that occurred in the mammalian target of rapamycin (mTOR) signaling pathway in the kidney as a result of deoxycorticosterone acetate (DOCA)-salt hypertension. Rats were implanted with DOCA strips (200 mg/kg) 1 week after unilateral nephrectomy and were then supplied with 0.9% saline to drink. Four weeks after DOCA implantation, systolic blood pressure (SBP) was measured by use of the tail-cuff method. The expression levels of phosphorylated phosphatidylinositol-3-kinase (PI3K), Akt, and mTOR, as well as the protein expression levels of ED-1 and cyclooxygenase-2 (COX-2), transforming growth factor-β1 (TGF-β1), α-smooth muscle actin (SMA), caspase-3, Bax, and Bcl-2, were then examined in the kidney by semiquantitative immunoblotting. DOCA-salt hypertensive rats were found to have significantly increased SBP as well as an increased kidney weight-to-body weight ratio. Moreover, the phosphorylation of PI3K, Akt, and mTOR was increased in the kidney of DOCA-salt hypertensive rats compared with the control, as was the protein expression of ED-1, COX-2, TGF-β1, and α-SMA. The expression levels of caspase-3 and Bax were increased significantly, whereas Bcl-2 expression was decreased. In conclusion, the phosphorylation of PI3K/Akt/mTOR was increased in the kidney of DOCA-salt hypertensive rats. Chonnam National University Medical School 2012-12 2012-12-21 /pmc/articles/PMC3539094/ /pubmed/23323219 http://dx.doi.org/10.4068/cmj.2012.48.3.150 Text en © Chonnam Medical Journal, 2012 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ma, Seong Kwon
Choi, Joon Seok
Joo, Soo Yeon
Kim, Ha Yeon
Kim, Chang Seong
Bae, Eun Hui
Lee, Jong Un
Kim, Soo Wan
Activation of the Renal PI3K/Akt/mTOR Signaling Pathway in a DOCA-Salt Model of Hypertension
title Activation of the Renal PI3K/Akt/mTOR Signaling Pathway in a DOCA-Salt Model of Hypertension
title_full Activation of the Renal PI3K/Akt/mTOR Signaling Pathway in a DOCA-Salt Model of Hypertension
title_fullStr Activation of the Renal PI3K/Akt/mTOR Signaling Pathway in a DOCA-Salt Model of Hypertension
title_full_unstemmed Activation of the Renal PI3K/Akt/mTOR Signaling Pathway in a DOCA-Salt Model of Hypertension
title_short Activation of the Renal PI3K/Akt/mTOR Signaling Pathway in a DOCA-Salt Model of Hypertension
title_sort activation of the renal pi3k/akt/mtor signaling pathway in a doca-salt model of hypertension
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539094/
https://www.ncbi.nlm.nih.gov/pubmed/23323219
http://dx.doi.org/10.4068/cmj.2012.48.3.150
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