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Functional multiplex reporter assay using tagged Gaussia luciferase

We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc(tag)), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc(tag) detection, this system...

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Detalles Bibliográficos
Autores principales: van Rijn, Sjoerd, Nilsson, Jonas, Noske, David P., Vandertop, W. Peter, Tannous, Bakhos A., Würdinger, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541509/
https://www.ncbi.nlm.nih.gov/pubmed/23308339
http://dx.doi.org/10.1038/srep01046