Functional multiplex reporter assay using tagged Gaussia luciferase

We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc(tag)), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc(tag) detection, this system...

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Detalles Bibliográficos
Autores principales: van Rijn, Sjoerd, Nilsson, Jonas, Noske, David P., Vandertop, W. Peter, Tannous, Bakhos A., Würdinger, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541509/
https://www.ncbi.nlm.nih.gov/pubmed/23308339
http://dx.doi.org/10.1038/srep01046
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author van Rijn, Sjoerd
Nilsson, Jonas
Noske, David P.
Vandertop, W. Peter
Tannous, Bakhos A.
Würdinger, Thomas
author_facet van Rijn, Sjoerd
Nilsson, Jonas
Noske, David P.
Vandertop, W. Peter
Tannous, Bakhos A.
Würdinger, Thomas
author_sort van Rijn, Sjoerd
collection PubMed
description We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc(tag)), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc(tag) detection, this system allowed the independent and real-time monitoring of mixed cell cultures in vitro and of mixed subcutaneous and intracranial tumor subpopulations in vivo.
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spelling pubmed-35415092013-01-10 Functional multiplex reporter assay using tagged Gaussia luciferase van Rijn, Sjoerd Nilsson, Jonas Noske, David P. Vandertop, W. Peter Tannous, Bakhos A. Würdinger, Thomas Sci Rep Article We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc(tag)), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc(tag) detection, this system allowed the independent and real-time monitoring of mixed cell cultures in vitro and of mixed subcutaneous and intracranial tumor subpopulations in vivo. Nature Publishing Group 2013-01-10 /pmc/articles/PMC3541509/ /pubmed/23308339 http://dx.doi.org/10.1038/srep01046 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
van Rijn, Sjoerd
Nilsson, Jonas
Noske, David P.
Vandertop, W. Peter
Tannous, Bakhos A.
Würdinger, Thomas
Functional multiplex reporter assay using tagged Gaussia luciferase
title Functional multiplex reporter assay using tagged Gaussia luciferase
title_full Functional multiplex reporter assay using tagged Gaussia luciferase
title_fullStr Functional multiplex reporter assay using tagged Gaussia luciferase
title_full_unstemmed Functional multiplex reporter assay using tagged Gaussia luciferase
title_short Functional multiplex reporter assay using tagged Gaussia luciferase
title_sort functional multiplex reporter assay using tagged gaussia luciferase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541509/
https://www.ncbi.nlm.nih.gov/pubmed/23308339
http://dx.doi.org/10.1038/srep01046
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