Cargando…

Functional multiplex reporter assay using tagged Gaussia luciferase

We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc(tag)), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc(tag) detection, this system...

Descripción completa

Detalles Bibliográficos
Autores principales:	van Rijn, Sjoerd, Nilsson, Jonas, Noske, David P., Vandertop, W. Peter, Tannous, Bakhos A., Würdinger, Thomas
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2013
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541509/ https://www.ncbi.nlm.nih.gov/pubmed/23308339 http://dx.doi.org/10.1038/srep01046

Ejemplares similares

Imaging Tumor Vascularity and Response to Anti-Angiogenic Therapy Using Gaussia Luciferase
por: Kantar, Rami S., et al.
Publicado: (2016)

Secreted Gaussia Luciferase as a Biomarker for Monitoring Tumor Progression and Treatment Response of Systemic Metastases
por: Chung, Euiheon, et al.
Publicado: (2009)

Secreted dual reporter assay with Gaussia luciferase and the red fluorescent protein mCherry
por: Wider, Diana, et al.
Publicado: (2017)

A reporter system for assaying influenza virus RNP functionality based on secreted Gaussia luciferase activity
por: Zhu, Wenfei, et al.
Publicado: (2011)

Gaussia Luciferase as a Reporter for Quorum Sensing in Staphylococcus aureus
por: Blower, Isobel, et al.
Publicado: (2020)

Virus replicon particle based Chikungunya virus neutralization assay using Gaussia luciferase as readout
por: Gläsker, Sabine, et al.
Publicado: (2013)

Membrane-bound Gaussia luciferase as a tool to track shedding of membrane proteins from the surface of extracellular vesicles
por: Zaborowski, Mikołaj Piotr, et al.
Publicado: (2019)

In Vivo Imaging of Ligand Receptor Binding with Gaussia Luciferase Complementation
por: Luker, Kathryn E., et al.
Publicado: (2011)

Application of Gaussia luciferase in bicistronic and non-conventional secretion reporter constructs
por: Luft, Christin, et al.
Publicado: (2014)

A Recombinant Porcine Reproductive and Respiratory Syndrome Virus Stably Expressing a Gaussia Luciferase for Antiviral Drug Screening Assay and Luciferase-Based Neutralization Assay
por: Li, Yanhua, et al.
Publicado: (2022)

A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer
por: Liu, Yang, et al.
Publicado: (2023)

Truncated Variants of Gaussia Luciferase with Tyrosine Linker for Site-Specific Bioconjugate Applications
por: Hunt, Eric A., et al.
Publicado: (2016)

A general method for quantifying ligand binding to unmodified receptors using Gaussia luciferase
por: Tóth, András Dávid, et al.
Publicado: (2021)

Systemically administered AAV9-sTRAIL combats invasive glioblastoma in a patient-derived orthotopic xenograft model
por: Crommentuijn, Matheus HW, et al.
Publicado: (2016)

Development of a highly sensitive Gaussia luciferase immunoprecipitation assay for the detection of antibodies against African swine fever virus
por: Ding, Jingjing, et al.
Publicado: (2022)

A Cytoplasmic Form of Gaussia luciferase Provides a Highly Sensitive Test for Cytotoxicity
por: Tsuji, Saori, et al.
Publicado: (2016)

Easy detection of hormone secretion from LβT2 cells by using Gaussia luciferase
por: SATOU, Kazuhiro, et al.
Publicado: (2017)

Development of recombinant rabies viruses vectors with Gaussia luciferase reporter based on Chinese vaccine strain CTN181
por: Du, Jialiang, et al.
Publicado: (2011)

Multiplexing fluorogenic esterase-based viability assay with luciferase assays
por: Ohgane, Kenji, et al.
Publicado: (2019)

Evaluation of Gaussia luciferase and foot-and-mouth disease virus 2A translational interrupter chimeras as polycistronic reporters for transgene expression
por: Puckette, Michael, et al.
Publicado: (2017)

Secreted Gaussia princeps Luciferase as a Reporter of Escherichia coli Replication in a Mouse Tissue Cage Model of Infection
por: Liu, Mingyu, et al.
Publicado: (2014)

A Simple and Robust Approach for Evaluation of Antivirals Using a Recombinant Influenza Virus Expressing Gaussia Luciferase
por: Li, Ping, et al.
Publicado: (2018)

Generation of a Reassortant Influenza A Subtype H3N2 Virus Expressing Gaussia Luciferase
por: Wang, Lin, et al.
Publicado: (2019)

Longitudinal monitoring of Gaussia and Nano luciferase activities to concurrently assess ER calcium homeostasis and ER stress in vivo
por: Wires, Emily S., et al.
Publicado: (2017)

Bioluminescence-mediated longitudinal monitoring of adipose-derived stem cells in a large mammal ex vivo organ culture
por: Peeters, Mirte, et al.
Publicado: (2015)

A Recombinant Genotype I Japanese Encephalitis Virus Expressing a Gaussia Luciferase Gene for Antiviral Drug Screening Assay and Neutralizing Antibodies Detection
por: Li, Chenxi, et al.
Publicado: (2022)

Listeria innocua Dps as a nanoplatform for bioluminescence based photodynamic therapy utilizing Gaussia princeps luciferase and zinc protoporphyrin IX
por: Al-Ani, Ali W., et al.
Publicado: (2019)

A Gaussia Luciferase Cell-Based System to Assess the Infection of Cell Culture- and Serum-Derived Hepatitis C Virus
por: Koutsoudakis, George, et al.
Publicado: (2012)

Solution structure of Gaussia Luciferase with five disulfide bonds and identification of a putative coelenterazine binding cavity by heteronuclear NMR
por: Wu, Nan, et al.
Publicado: (2020)

EFEMP1 induces γ-secretase/Notch-mediated temozolomide resistance in glioblastoma
por: Hiddingh, Lotte, et al.
Publicado: (2013)

A codon-optimized luciferase from Gaussia princeps facilitates the in vivo monitoring of gene expression in the model alga Chlamydomonas reinhardtii
por: Shao, Ning, et al.
Publicado: (2008)

Targeted Bioluminescent Imaging of Pancreatic Ductal Adenocarcinoma Using Nanocarrier-Complexed EGFR-Binding Affibody–Gaussia Luciferase Fusion Protein
por: Hersh, Jessica, et al.
Publicado: (2023)

Genetically modified cancer models: the value of luciferase-tagged tumors and in vivo luciferase-based reporter assays in oncology drug discovery
por: Yang, W, et al.
Publicado: (2003)

Minicircle HBV cccDNA with a Gaussia luciferase reporter for investigating HBV cccDNA biology and developing cccDNA-targeting drugs
por: Li, Feng, et al.
Publicado: (2016)

Rapid and sensitive large-scale screening of low affinity extracellular receptor protein interactions by using reaction induced inhibition of Gaussia luciferase
por: Galaway, Francis, et al.
Publicado: (2020)

Influenza A Virus Encoding Secreted Gaussia Luciferase as Useful Tool to Analyze Viral Replication and Its Inhibition by Antiviral Compounds and Cellular Proteins
por: Eckert, Nadine, et al.
Publicado: (2014)

A new humanized in vivo model of KIT D816V(+) advanced systemic mastocytosis monitored using a secreted luciferase
por: Bibi, Siham, et al.
Publicado: (2016)

Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying
por: Sarrion-Perdigones, Alejandro, et al.
Publicado: (2019)

A chemical screen for medulloblastoma identifies quercetin as a putative radiosensitizer
por: Lagerweij, Tonny, et al.
Publicado: (2016)

A simple in vitro tumor chemosensitivity assay based on cell penetrating peptide tagged luciferase
por: Yu, Tingyu, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_i0o6ps23shv2meom47shd2mal1