A Homogeneous, High-Throughput Assay for Phosphatidylinositol 5-Phosphate 4-Kinase with a Novel, Rapid Substrate Preparation

Phosphoinositide kinases regulate diverse cellular functions and are important targets for therapeutic development for diseases, such as diabetes and cancer. Preparation of the lipid substrate is crucial for the development of a robust and miniaturizable lipid kinase assay. Enzymatic assays for phos...

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Detalles Bibliográficos
Autores principales: Davis, Mindy I., Sasaki, Atsuo T., Shen, Min, Emerling, Brooke M., Thorne, Natasha, Michael, Sam, Pragani, Rajan, Boxer, Matthew, Sumita, Kazutaka, Takeuchi, Koh, Auld, Douglas S., Li, Zhuyin, Cantley, Lewis C., Simeonov, Anton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542272/
https://www.ncbi.nlm.nih.gov/pubmed/23326584
http://dx.doi.org/10.1371/journal.pone.0054127

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542272/
https://www.ncbi.nlm.nih.gov/pubmed/23326584
http://dx.doi.org/10.1371/journal.pone.0054127

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