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Condensation of an Additive-Free Cell Extract to Mimic the Conditions of Live Cells

The cellular environment differs from that of reconstituted materials mainly because of the presence of highly condensed biomacromolecules. To mimic the environment and conditions in living cells, we developed a method to prepare additive-free, highly concentrated cell extracts. First, we verified t...

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Detalles Bibliográficos
Autores principales: Fujiwara, Kei, Nomura, Shin-ichiro M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542322/
https://www.ncbi.nlm.nih.gov/pubmed/23326590
http://dx.doi.org/10.1371/journal.pone.0054155