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Condensation of an Additive-Free Cell Extract to Mimic the Conditions of Live Cells
The cellular environment differs from that of reconstituted materials mainly because of the presence of highly condensed biomacromolecules. To mimic the environment and conditions in living cells, we developed a method to prepare additive-free, highly concentrated cell extracts. First, we verified t...
Autores principales: | Fujiwara, Kei, Nomura, Shin-ichiro M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542322/ https://www.ncbi.nlm.nih.gov/pubmed/23326590 http://dx.doi.org/10.1371/journal.pone.0054155 |
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