Optimization of Taq DNA polymerase enzyme expression in Escherichia coli

BACKGROUND: In the present study, we optimized the experimental conditions using pET15b expression vector to obtain large amounts of Taq DNA polymerase. MATERIALS AND METHODS: Correct framing of the gene in the expression vector pET15b and its orientation were analyzed by digestion and sequencing. P...

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Detalles Bibliográficos
Autores principales: Moazen, Fateme, Rastegari, Ali, Hoseini, Sayed Mehdi, Panjehpour, Mojtaba, Miroliaei, Mehran, Sadeghi, Hamid Mir Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544109/
https://www.ncbi.nlm.nih.gov/pubmed/23326812
http://dx.doi.org/10.4103/2277-9175.103004

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544109/
https://www.ncbi.nlm.nih.gov/pubmed/23326812
http://dx.doi.org/10.4103/2277-9175.103004

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