Cargando…

Optimization of Taq DNA polymerase enzyme expression in Escherichia coli

BACKGROUND: In the present study, we optimized the experimental conditions using pET15b expression vector to obtain large amounts of Taq DNA polymerase. MATERIALS AND METHODS: Correct framing of the gene in the expression vector pET15b and its orientation were analyzed by digestion and sequencing. P...

Descripción completa

Detalles Bibliográficos
Autores principales:	Moazen, Fateme, Rastegari, Ali, Hoseini, Sayed Mehdi, Panjehpour, Mojtaba, Miroliaei, Mehran, Sadeghi, Hamid Mir Mohammad
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Medknow Publications & Media Pvt Ltd 2012
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544109/ https://www.ncbi.nlm.nih.gov/pubmed/23326812 http://dx.doi.org/10.4103/2277-9175.103004

Ejemplares similares

Mutating Asn-666 to Glu in the O-helix region of the taq DNA polymerase gene
por: Sadeghi, H. Mir Mohammad, et al.
Publicado: (2010)

Optimization of the Expression of Reteplase in Escherichia coli TOP10 Using Arabinose Promoter
por: Shafiee, Fatemeh, et al.
Publicado: (2015)

Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes
por: Tajadini, Mohamadhasan, et al.
Publicado: (2014)

Optimization of the expression of reteplase in Escherichia coli
por: Sadeghi, H. Mir Mohammad, et al.
Publicado: (2011)

Optimization of the expression of phaC2 encoding poly (3-hydroxyalkanoate) synthase from Pseudomonas aeruginosa PTCC1310 in Fad B deleted Escherichia coli
por: Abedi, Daryoush, et al.
Publicado: (2016)

Development of a Simple Direct and Hot-Start PCR Using Escherichia coli-Expressing Taq DNA Polymerase
por: Lee, Sun Ju, et al.
Publicado: (2023)

Surface Engineering of Escherichia coli–Derived OMVs as Promising Nano-Carriers to Target EGFR-Overexpressing Breast Cancer Cells
por: Sepahdar, Zahra, et al.
Publicado: (2021)

Inhibitory effects of selected isoquinoline alkaloids against main protease (M(pro)) of SARS-CoV-2, in silico study
por: Sadeghi, Morteza, et al.
Publicado: (2022)

Optimization of Fermentation Conditions for Reteplase Expression by Escherichia coli Using Response Surface Methodology
por: Zare, Hamze, et al.
Publicado: (2019)

Bst polymerase — a humble relative of Taq polymerase
por: Oscorbin, Igor, et al.
Publicado: (2023)

Improvement of Soluble Production of Reteplase in Escherichia coli by Optimization of Chemical Chaperones in Lysis Buffer
por: Fazeli, Behnaz, et al.
Publicado: (2019)

Polymerase chain reaction amplification of a GC rich region by adding 1,2 propanediol
por: Mousavian, Zeinab, et al.
Publicado: (2014)

Single-molecule Taq DNA polymerase dynamics
por: Turvey, Mackenzie W., et al.
Publicado: (2022)

Inhibition of Glycation-induced Cytotoxicity, Protein Glycation, and Activity of Proteolytic Enzymes by Extract from Perovskia atriplicifolia Roots
por: Miroliaei, Mehran, et al.
Publicado: (2017)

Data of expression and purification of recombinant Taq DNA polymerase
por: Fang, Na, et al.
Publicado: (2016)

Effect of Twine-arginine Translocation-signaling Fusion System and Chaperones Co-expression on Secretory Expression of Somatropin
por: Bagherinejad, Mohammad Reza, et al.
Publicado: (2018)

Cloning and Expression of Randomly Mutated Bacillus subtilis α-Amylase Genes in HB101
por: Rabbani, Mohammed, et al.
Publicado: (2011)

Optimization of the Expression of Genes Encoding Poly (3-hydroxyalkanoate) Synthase from Pseudomonas aeruginosa PTCC 1310 in Escherichia coli
por: Abedi, Daryoush, et al.
Publicado: (2012)

Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients
por: Rastegari, Ali, et al.
Publicado: (2015)

Optimizing Taq Polymerase Concentration for Improved Signal-to-Noise in the Broad Range Detection of Low Abundance Bacteria
por: Spangler, Rudolph, et al.
Publicado: (2009)

Cloning and Expression of Functional Reteplase in Escherichia coli TOP10
por: Khodabakhsh, Fatemeh, et al.
Publicado: (2013)

The influence of BsmI and TaqI vitamin D receptor gene polymorphisms on the intensity of hyperparathyroidism in Iranian hemodialysis patients
por: Pourfarzam, Morteza, et al.
Publicado: (2014)

Translocation and fidelity of Escherichia coli RNA polymerase
por: Nedialkov, Yuri A., et al.
Publicado: (2013)

ENZYME BIOSYNTHESIS IN ESCHERICHIA COLI
por: Weinbaum, George, et al.
Publicado: (1959)

Data of self-made Taq DNA polymerase prepared for screening purposes
por: Konovalova, E.V., et al.
Publicado: (2017)

Cloning, Optimization of Periplasmic Expression and Purification of Recombinant Granulocyte Macrophage-Stimulating Factor in Escherichia coli BL21 (DE3)
por: Taherian, Elham, et al.
Publicado: (2019)

Optimization of a single-chain antibody fragment overexpression in Escherichia coli using response surface methodology
por: Akbari, V., et al.
Publicado: (2015)

Phenotypic consequences of RNA polymerase dysregulation in Escherichia coli
por: Sarkar, Paramita, et al.
Publicado: (2017)

Co-Expression of Chaperones for Improvement of Soluble Expression and Purification of An Anti-HER2 scFv in Escherichia Coli
por: Estabragh, Amir Mirzapour, et al.
Publicado: (2022)

Widespread antibiotic resistance of diarrheagenic Escherichia coli and Shigella species
por: Sadeghabadi, Azam Fatahi, et al.
Publicado: (2014)

Optimization of solvent media to solubilize TEV protease using response surface method
por: Mohammadian, Niloufar, et al.
Publicado: (2020)

Switching the activity of Taq polymerase using clamp-like triplex aptamer structure
por: Hu, Yingxin, et al.
Publicado: (2020)

Improvement of isoprene production in Escherichia coli by rational optimization of RBSs and key enzymes screening
por: Li, Meijie, et al.
Publicado: (2019)

DNA polymerase switching: effects on spontaneous mutagenesis in Escherichia coli
por: Curti, Elena, et al.
Publicado: (2009)

Activity Map of the Escherichia coli RNA Polymerase Bridge Helix
por: Jovanovic, Milija, et al.
Publicado: (2011)

Modified Taq DNA Polymerase for Allele-Specific Ultra-Sensitive Detection of Genetic Variants
por: Lim, Youngshin, et al.
Publicado: (2022)

Codon optimization of a gene encoding DNA polymerase from Pyrococcus furiosus and its expression in Escherichia coli
por: Nuryana, Isa, et al.
Publicado: (2023)

Chemical Investigations in Kelussia odoratissima Mozaff. Leaves Based on Comprehensive Analytical Methods: LC-MS, SPME, and GC-MS Analyses
por: Rahimmalek, Mehdi, et al.
Publicado: (2023)

Novel properties of recombinant Sso7d-Taq DNA polymerase purified using aqueous two-phase extraction: Utilities of the enzyme in viral diagnosis
por: Sundarrajan, Sudarson, et al.
Publicado: (2018)

Rosmarinic acid inhibits DNA glycation and modulates the expression of Akt1 and Akt3 partially in the hippocampus of diabetic rats
por: Alrubaye, Ameer, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_ou8vkk9hukbpft1acd0eeimu86