A novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion

Stem cell factor (SCF) activates hematopoietic stem cell (HSC) self-renewal and is being used to stimulate the ex vivo expansion of HSCs. The mechanism by which SCF supports expansion of HSCs remains poorly understood. In cord blood ex vivo expansion assays, a newly produced anti-SCF monoclonal anti...

Descripción completa

Detalles Bibliográficos
Autores principales: Fan, Jie, Ding, Xinxin, Jiang, Yongping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544603/
https://www.ncbi.nlm.nih.gov/pubmed/23228140
http://dx.doi.org/10.1186/1756-8722-5-73
_version_ 1782255805115924480
author Fan, Jie
Ding, Xinxin
Jiang, Yongping
author_facet Fan, Jie
Ding, Xinxin
Jiang, Yongping
author_sort Fan, Jie
collection PubMed
description Stem cell factor (SCF) activates hematopoietic stem cell (HSC) self-renewal and is being used to stimulate the ex vivo expansion of HSCs. The mechanism by which SCF supports expansion of HSCs remains poorly understood. In cord blood ex vivo expansion assays, a newly produced anti-SCF monoclonal antibody (clone 23C8) was found to significantly inhibit the expansion of CD34(+) cells. This antibody appears to bind directly to a part of SCF that is critical for biological activity toward expansion of CD34(+) cells, which is located in the first 104 amino acids from the NH(2)-terminus.
format Online
Article
Text
id pubmed-3544603
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-35446032013-01-16 A novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion Fan, Jie Ding, Xinxin Jiang, Yongping J Hematol Oncol Letter to the Editor Stem cell factor (SCF) activates hematopoietic stem cell (HSC) self-renewal and is being used to stimulate the ex vivo expansion of HSCs. The mechanism by which SCF supports expansion of HSCs remains poorly understood. In cord blood ex vivo expansion assays, a newly produced anti-SCF monoclonal antibody (clone 23C8) was found to significantly inhibit the expansion of CD34(+) cells. This antibody appears to bind directly to a part of SCF that is critical for biological activity toward expansion of CD34(+) cells, which is located in the first 104 amino acids from the NH(2)-terminus. BioMed Central 2012-12-10 /pmc/articles/PMC3544603/ /pubmed/23228140 http://dx.doi.org/10.1186/1756-8722-5-73 Text en Copyright ©2012 Fan et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Letter to the Editor
Fan, Jie
Ding, Xinxin
Jiang, Yongping
A novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion
title A novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion
title_full A novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion
title_fullStr A novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion
title_full_unstemmed A novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion
title_short A novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion
title_sort novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544603/
https://www.ncbi.nlm.nih.gov/pubmed/23228140
http://dx.doi.org/10.1186/1756-8722-5-73
work_keys_str_mv AT fanjie anovelmonoclonalantibodyofhumanstemcellfactorinhibitsumbilicalcordbloodstemcellexvivoexpansion
AT dingxinxin anovelmonoclonalantibodyofhumanstemcellfactorinhibitsumbilicalcordbloodstemcellexvivoexpansion
AT jiangyongping anovelmonoclonalantibodyofhumanstemcellfactorinhibitsumbilicalcordbloodstemcellexvivoexpansion
AT fanjie novelmonoclonalantibodyofhumanstemcellfactorinhibitsumbilicalcordbloodstemcellexvivoexpansion
AT dingxinxin novelmonoclonalantibodyofhumanstemcellfactorinhibitsumbilicalcordbloodstemcellexvivoexpansion
AT jiangyongping novelmonoclonalantibodyofhumanstemcellfactorinhibitsumbilicalcordbloodstemcellexvivoexpansion

Ejemplares similares