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Protocol: Chromatin immunoprecipitation (ChIP) methodology to investigate histone modifications in two model diatom species

In this report we describe a chromatin immunoprecipitation (ChIP) protocol for two fully sequenced model diatom species Phaeodactylum tricornutum and Thalassiosira pseudonana. This protocol allows the extraction of satisfactory amounts of chromatin and gives reproducible results. We coupled the ChIP...

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Detalles Bibliográficos
Autores principales: Lin, Xin, Tirichine, Leïla, Bowler, Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546051/
https://www.ncbi.nlm.nih.gov/pubmed/23217141
http://dx.doi.org/10.1186/1746-4811-8-48